Atomic-Scale Corrugations in Crystalline Polypeptoid Nanosheets Revealed by Three-Dimensional Cryogenic Electron Microscopy

Amphiphilic molecules that can crystallize often form molecularly thin nanosheets in aqueous solutions. The possibility of atomic-scale corrugations in these structures has not yet been recognized. We have studied the self-assembly of amphiphilic polypeptoids, a family of bio-inspired polymers that can self-assemble into various crystalline nanostructures. Atomic-scale structure of the crystals in these systems has been inferred using both X-ray diffraction and electron microscopy. Here we use cryogenic electron microscopy to determine the in-plane and out-of-plane structures of a crystalline nanosheet. Data were collected as a function of tilt angle and analyzed using a hybrid single-particle crystallographic approach. The analysis reveals that adjacent rows of peptoid chains, which are separated by 4.5 angstrom in the plane of the nanosheet, are offset by 6 angstrom in the direction perpendicular to the plane of the nanosheet. These atomic-scale corrugations lead to a doubling of the unit cell dimension from 4.5 to 9 angstrom. Our work provides an alternative interpretation for the observed angstrom X-ray diffraction peak often reported in polypeptoid crystals.

Automated summary

Amphiphilic molecules in aqueous solutions can form molecularly thin nanosheets. This study examines the atomic-scale structure of crystalline nanosheets formed by amphiphilic polypeptoids using cryogenic electron microscopy. The analysis reveals atomic-scale corrugations in the nanosheet, resulting in a doubling of the unit cell dimension. This work provides an alternative interpretation for observed angstrom X-ray diffraction peaks in polypeptoid crystals.