Hydrazone-Based Amphiphilic Brush Polymer for Fast Endocytosis and ROS-Active Drug Release

Due to the high reactivity of reactive oxygen species (ROS), it is essential to sweep them away in time. In this study, ClO--responsible amphiphilic brush polymers were prepared by free radical polymerization using two monomers consisting of polyethylene glycol as the hydrophilic part, and an alkyl chain connected by hydrazone as the hydrophobic part. The macromolecules assemble into particles with nanoscaled dimensions in a neutral buffer, which ensures quick cellular internalization. The polymer has a low critical micellization concentration and can encapsulate hydrophobic drug molecules up to 19% wt. The micelles formed by the polymer disassemble in a ClO--rich environment and release 80% of their cargo within 2 h, which possesses a faster release rate compared to the previous systems. The relatively small size and the quick response of hydrazone toward ClO- ensure a quick uptake and elimination of ROS in vitro and in vivo.

Automated summary

In this study, ClO--sensitive amphiphilic brush polymers were synthesized, which can self-assemble into nano-sized particles in a neutral buffer, allowing for rapid cellular uptake. These polymers have a low critical micellization concentration and can encapsulate hydrophobic drug molecules up to 19% wt. The micelles disassemble in a ClO--rich environment and exhibit a faster drug release rate compared to previous systems, ensuring quick ROS elimination in vitro and in vivo.