Accelerated degradation of plastic products via yeast enzyme treatment

Biodegradable plastics can solve the problem of unwanted plastics accumulating in the environment if they can be given the contradictory properties of durability in use and rapid degradation after use. Commercially available agricultural biodegradable mulch films are made from formulations containing polybutylene adipate-co-terephthalate (PBAT) to provide mechanical and UV resistance during the growing season. Although used films are ploughed into the soil using a tiller to promote decomposition, it is difficult if they remain durable. We showed that an enzyme produced by the leaf surface yeast Pseudozyma antarctica (PaE) degrades PBAT-containing films. In laboratory studies, PaE randomly cleaved the PBAT polymer chain and induced erosion of the film surface. In the field, commercial biodegradable films containing PBAT placed on ridges were weakened in both the warm and cold seasons by spraying the culture filtrate of P. antarctica. After the field was ploughed the next day, the size and total weight of residual film fragments decreased significantly (p < 0.05). Durable biodegradable plastics used in the field are degraded using PaE treatment and are broken down into small fragments by the plough. The resultant degradation products can then be more readily assimilated by many soil microorganisms.

Automated summary

If biodegradable plastics can combine durability in use with rapid degradation after use, they have the potential to solve the problem of environmental plastic accumulation. We have discovered that an enzyme produced by the leaf surface yeast Pseudozyma antarctica (PaE) can degrade films containing polybutylene adipate-co-terephthalate (PBAT). In field experiments, biodegradable films containing PBAT were weakened and reduced in size and weight through PaE treatment and ploughing.