Journal
SCIENTIFIC REPORTS
Volume 13, Issue 1, Pages -Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41598-023-29396-0
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Functionalization of graphene is a crucial technology in various fields. We have developed an oxidative modification method using photoactivated ClO2 as a mild oxidant, which allows the graphene to retain its functionality for at least three months. By chemically functionalizing the graphene, we created an epoxidized graphene grid (EG-grid), which greatly improves the density and orientation distribution of protein particles for cryoEM analysis. The EG-grid enables atomic-resolution imaging and highly efficient high-resolution cryoEM structural analysis of biological macromolecules.
Functionalization of graphene is one of the most important fundamental technologies in a wide variety of fields including industry and biochemistry. We have successfully achieved a novel oxidative modification of graphene using photoactivated ClO2 as a mild oxidant and confirmed the oxidized graphene grid is storable with its functionality for at least three months under N-2 atmosphere. Subsequent chemical functionalization enabled us to develop an epoxidized graphene grid (EG-grid (TM)), which effectively adsorbs protein particles for electron cryomicroscopy (cryoEM) image analysis. The EG-grid dramatically improved the particle density and orientation distribution. The density maps of GroEL and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were reconstructed at 1.99 and 2.16 angstrom resolution from only 504 and 241 micrographs, respectively. A sample solution of 0.1 mg ml(-1) was sufficient to reconstruct a 3.10 angstrom resolution map of SARS-CoV-2 spike protein from 1163 micrographs. The map resolutions of beta-galactosidase and apoferritin easily reached 1.81 angstrom and 1.29 angstrom resolution, respectively, indicating its atomic-resolution imaging capability. Thus, the EG-grid will be an extremely powerful tool for highly efficient high-resolution cryoEM structural analysis of biological macromolecules.
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