Spirulina platensis improves growth, oil content, and antioxidant activitiy of rosemary plant under cadmium and lead stress

In the present study, a pot experiment was conducted to investigate the response of rosemary (Rosmarinus officinalis L.) plants to foliar application of Spirulina platensis at 0.0, 0.1, 0.2, and 0.4%; soil irrigation with heavy metals (Cd nitrate, Pb acetate, and Cd + Pb, each at 100 ppm), and Spirulina platensis at 0.1% + heavy metals. Spirulina platensis significantly improved growth parameters, oil yield/fed, photosynthetic pigments, and activity of superoxide dismutase (SOD), glutathione reductase (GR), catalase (CAT), and polyphenol oxidase (PPO) with a maximum promoting effect at 0.2% algal extract. On the other hand, heavy metal stress reduced growth criteria, photosynthetic pigments, and oil yield, while, significantly increased levels of antioxidant enzymes (SOD, CAT, GR) and corresponding non-enzymatic antioxidants (ascorbic acid, total antioxidant capacity, phenolics and flavonoids). Bioaccumulation factor (BF) and translocation factor (TF) indicated that Cd and Pb accumulated largely in the roots, with little transfer to the shoots. Nevertheless, compared with heavy metal treatments, S. platensis at 0.1% significantly increasing growth parameters, oil content, photosynthetic pigments, and the activity of non-enzymatic and enzymatic antioxidants, while, slightly reduced TF of Cd and Pb, alleviated membrane lipid peroxidation, and significantly lowered the content of malondialdehyde, hydrogen peroxide, and indole acetic acid oxidase (IAAO) activity in heavy metal (Cd, Pb, and Cd + Pb)-treated rosemary plants.

Automated summary

This study investigated the effects of foliar application of Spirulina platensis and soil irrigation with heavy metals on the growth and antioxidant activity of rosemary plants. Results showed that Spirulina platensis significantly improved plant growth, oil yield, photosynthetic pigments, and antioxidant enzyme activity. Conversely, heavy metal stress negatively affected growth and oil yield but increased antioxidant enzyme levels. Analysis of bioaccumulation and translocation factors revealed that Cd and Pb accumulated primarily in the roots. However, the addition of 0.1% Spirulina platensis mitigated the negative effects of heavy metals, improving growth parameters, antioxidant activity, and reducing lipid peroxidation and oxidative stress markers in rosemary plants.