4.7 Article

Optimized protocol for MALDI MSI of N-glycans using an on-tissue digestion in fresh frozen tissue sections

Related references

Note: Only part of the references are listed.
Article Biochemical Research Methods

An optimized MALDI MSI protocol for spatial detection of tryptic peptides in fresh frozen prostate tissue

Therese S. Hoiem et al.

Summary: MALDI MS imaging is a powerful tool for spatial peptide detection in heterogeneous tissues, and proper sample preparation is crucial for high-quality measurements. In this study, an optimized protocol was developed for spatially resolved proteolytic peptide detection in fresh frozen prostate tissue sections using MALDI time-of-flight MSI.

PROTEOMICS (2022)

Article Oncology

High-Mannose N-Glycans as Malignant Progression Markers in Early-Stage Colorectal Cancer

Fanny Boyaval et al.

Summary: This study investigates the changes in sugar molecules during the progression of colorectal cancer (CRC) and finds that these sugar signatures can clearly differentiate different stages of CRC progression, especially between pre-cancerous and cancerous tissue. The study also observes the spreading of glycosylation signatures into the tumor microenvironment.

CANCERS (2022)

Article Biochemical Research Methods

Controlled Humidity Levels for Fine Spatial Detail Information in Enzyme-Assisted N-Glycan MALDI MSI

Dusan Velickovic et al.

Summary: Investigation of the spatial distribution of N-glycans in tissue specimens is important in clinical research as altered N-glycans are often indicative of disease progression. By optimizing the incubation step, accurate and sensitive MALDI-MSI of N-glycans was achieved, allowing for the identification of distinct spatial patterns in tissue sections.

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY (2022)

Article Biochemical Research Methods

Mass spectrometry imaging spatially identifies complex-type N-glycans as putative cartilage degradation markers in human knee osteoarthritis tissue

Yea-Rin Lee et al.

Summary: This study utilized MALDI-MSI and LC-MS/MS to spatially map and identify specific degraded cartilage N-glycans in knee osteoarthritis patients for the first time. The identified N-glycans could potentially serve as diagnostic and prognostic biomarkers.

ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2022)

Review Biochemistry & Molecular Biology

N-glycans as functional effectors of genetic and epigenetic disease risk

Tamara Stambuk et al.

Summary: N-glycosylation is a crucial protein modification process that is influenced by various factors, including gene polymorphisms and epigenetic regulation. This process plays a significant role in multiple biological processes, with specific glycan structure modifications affecting protein biological functions.

MOLECULAR ASPECTS OF MEDICINE (2021)

Review Urology & Nephrology

Glycosylation in health and disease

Colin Reily et al.

NATURE REVIEWS NEPHROLOGY (2019)

Article Biochemical Research Methods

N-Linked Glycan Branching and Fucosylation Are Increased Directly in Hcc Tissue As Determined through in Situ Glycan Imaging

Connor A. West et al.

JOURNAL OF PROTEOME RESEARCH (2018)

Review Chemistry, Analytical

Mass Spectrometry Imaging: A Review of Emerging Advancements and Future Insights

Amanda Rae Buchberger et al.

ANALYTICAL CHEMISTRY (2018)

Review Biochemistry & Molecular Biology

Biological roles of glycans

Ajit Varki

GLYCOBIOLOGY (2017)

Review Biochemical Research Methods

A concise review of mass spectrometry imaging

Erika R. Amstalden van Hove et al.

JOURNAL OF CHROMATOGRAPHY A (2010)

Article Pathology

Citraconic anhydride: a new antigen retrieval solution

Anthony S-Y. Leong et al.

PATHOLOGY (2010)

Letter Biochemical Research Methods

mMass data miner: an open source alternative for mass spectrometric data analysis

Martin Strohalm et al.

RAPID COMMUNICATIONS IN MASS SPECTROMETRY (2008)

Review Biochemistry & Molecular Biology

Glycosylation in cellular mechanisms of health and disease

Kazuaki Ohtsubo et al.