IRF4 as a novel target involved in malignant transformation of oral submucous fibrosis into oral squamous cell carcinoma

Oral squamous cell carcinoma (OSCC) in the context of oral submucous fibrosis (OSF) has a high incidence owing to undefined pathogenesis. Identifying key genes and exploring the underlying molecular mechanisms involved in the conversion of OSF into OSCC are in urgent need. Differentially expressed genes (DEGs) between OSCC and OSF were dug from GEO databases and a total of 170 DEGs were acquired. Functional association of DEGs were analyzed by GO and KEGG. Protein-protein interactions (PPIs) analysis was carried out and candidate biomarkers were identified by Gene co-expression analysis and Cox analyses. Hub genes were confirmed by qRT-PCR in tissues and cell lines, of which we found that IRF4 mRNA was successively up-regulated from Normal to OSF and then to OSCC and associated with immune infiltrating levels. In addition, Immunohistochemical (IHC) and Immunofluorescence (IF) assays were conducted to validate the consistent upregulation of IRF4 and the oncogene role of IRF4 in OSF and OSCC at translation level. IRF4 may be indicative biomarker in transformation of OSF into OSCC. High IRF4 expression contribute to increased immune infiltration of OSCC and may provide a novel diagnostic marker for OSCC patients translated from OSF.

Automated summary

Oral squamous cell carcinoma (OSCC) has a high incidence in the presence of oral submucous fibrosis (OSF) due to unknown causes. This study aims to identify key genes and explore the molecular mechanisms involved in the conversion of OSF into OSCC. Through analysis of differentially expressed genes (DEGs) between OSCC and OSF, a total of 170 DEGs were identified. Further analyses revealed the functional associations, protein-protein interactions, and potential biomarkers. The overexpression of IRF4 was found to be associated with the progression from OSF to OSCC, and it may serve as a diagnostic marker and contribute to increased immune infiltration in OSCC.