Journal
CHEMBIOCHEM
Volume 17, Issue 2, Pages 159-163Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201500603
Keywords
biosensors; DNAzymes; organic solvents; RNA cleavage; sodium
Funding
- University of Waterloo
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Foundation for Shenghua Scholar of Central South University
- National Natural Science Foundation of China [21301195]
- China Scholarship Council (CSC) [201406370116]
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Enzymes working in organic solvents are important for analytical chemistry, catalysis, and mechanistic studies. Although a few protein enzymes are highly active in organic solvents, little is known regarding nucleic acid-based enzymes. Herein, we report the first RNA-cleaving DNAzyme, named EtNa, that works optimally in concentrated organic solvents containing only monovalent Na+. The EtNa DNAzyme has a rate of 2.0 h(-1) in 54% ethanol (with 120 mm NaCl and no divalent metal ions), and a Kd of 21 mm Na+. It retains activity even in 72% ethanol as well as in DMSO. With 4 mm Na+, the rate in 54% ethanol is >1000-fold higher than that in water. We also demonstrated the use of EtNa to measuring the ethanol content in alcoholic drinks. In total, this DNAzyme has three unique features: divalent metal independent activity, Na+ selectivity among monovalent metals, and acceleration by organic solvents.
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