Optofluidic adaptive optics in multi-photon microscopy

Adaptive optics, in combination with multi-photon techniques, is a powerful approach to image deep into a specimen. Remarkably, virtually all adaptive optics schemes today rely on wavefront modulators that are reflective, diffractive or both. This, however, can pose a severe limitation for applications. Here, we present a fast and robust sensorless adaptive optics scheme adapted for transmissive wavefront modulators. We study our scheme in numerical simulations and in experiments with a novel, optofluidic wavefront shaping device that is transmissive, refractive, polarisation-independent, and broadband. We demonstrate scatter correction of two-photon-excited fluorescence images of microbeads as well as brain cells and benchmark our device against a liquid-crystal spatial light modulator. Our method and technology could open new routes for adaptive optics in scenarios where previously, the restriction to reflective and diffractive devices may have staggered innovation and progress.Published by Optica Publishing Group under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article's title, journal citation, and DOI.

Automated summary

Adaptive optics combined with multi-photon techniques allows for deep imaging of specimens. However, most current adaptive optics schemes rely on reflective or diffractive wavefront modulators, which can be a limitation. In this study, we present a sensorless adaptive optics scheme that is adapted for transmissive wavefront modulators. We demonstrate its effectiveness in scatter correction of two-photon-excited fluorescence images and benchmark it against a liquid-crystal spatial light modulator.