Adaptive optics for optical microscopy [Invited]

Optical microscopy is widely used to visualize fine structures. When applied to bioimaging, its performance is often degraded by sample-induced aberrations. In recent years, adaptive optics (AO), originally developed to correct for atmosphere-associated aberrations, has been applied to a wide range of microscopy modalities, enabling high-or super-resolution imaging of biological structure and function in complex tissues. Here, we review classic and recently developed AO techniques and their applications in optical microscopy.

Automated summary

Optical microscopy is widely used, but its performance for bioimaging is often affected by sample-induced aberrations. Recently, adaptive optics (AO) techniques have been applied to microscopy, allowing high-resolution imaging of biological structures in complex tissues. This review discusses classic and recently developed AO techniques and their applications in optical microscopy.