ExpressAnalyst: A unified platform for RNA-sequencing analysis in non-model species

The increasing application of RNA sequencing to study non-model species demands easy-to-use and efficient bioinformatics tools to help researchers quickly uncover biological and functional insights. We developed ExpressAnalyst (www.expressanalyst.ca), a web-based platform for processing, analyzing, and interpreting RNA-sequencing data from any eukaryotic species. ExpressAnalyst contains a series of modules that cover from processing and annotation of FASTQ files to statistical and functional analysis of count tables or gene lists. All modules are integrated with EcoOmicsDB, an ortholog database that enables comprehensive analysis for species without a reference transcriptome. By coupling ultra-fast read mapping algorithms with highresolution ortholog databases through a user-friendly web interface, ExpressAnalyst allows researchers to obtain global expression profiles and gene-level insights from raw RNA-sequencing reads within 24 h. Here, we present ExpressAnalyst and demonstrate its utility with a case study of RNA-sequencing data from multiple non-model salamander species, including two that do not have a reference transcriptome.

Automated summary

ExpressAnalyst is a web-based platform that efficiently processes and analyzes RNA-sequencing data from any eukaryotic species, providing statistical and functional analysis. It includes modules for processing and annotating FASTQ files, as well as analyzing count tables or gene lists. By integrating ultra-fast read mapping algorithms with high-resolution ortholog databases, ExpressAnalyst enables researchers to obtain global expression profiles and gene-level insights within 24 h.