Journal
KIDNEY & BLOOD PRESSURE RESEARCH
Volume 41, Issue 2, Pages 119-128Publisher
KARGER
DOI: 10.1159/000443413
Keywords
Mesenchymal stromal cells; Extracellular vesicles; Acute kidney injury; Ischemia-reperfusion injury; Nrf2; HO-1
Funding
- National Natural Science Foundation of China [81170642, 81470919, 81370849, 81300472, 81202034]
- Natural Science Foundation of Jiangsu Province [BL2013032, BK2012336]
- high-level talents in six industries of Jiangsu Province [2013-WSW-052]
- Fundamental Research Funds for the Central Universities of China [3290005420]
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Background/Aims: Anti-oxidation is an effective strategy for curing acute kidney injury (AKI). Herein, we suggest that extracellular vesicles (EVs) derived from mesenchymal stromal cells (MSCs) might play an anti-oxidative role by enhancing Nrf2/ARE activation in AKI. Methods: EVs isolated from the conditioned medium of human Wharton's Jelly mesenchymal stromal cells and human foreskin fibroblast were intravenously injected in rats immediately after 45 min of unilateral kidney ischemia. Animals were sacrificed 24 h after injury. Results: Results showed that renal tubular injury was alleviated and renal function was improved by MSC-EVs. Cell apoptosis and sNGAL levels, which reflect kidney cell injury, were reduced. Moreover, MSC-EVs decreased oxidative stress in injured kidney tissues and NRK-52E cells under hypoxia injury. Nrf2/antioxidant response element (ARE) enhancement and HO-1 up-regulation were further observed after MSC-EV treatment both in vivo and in vitro. Conclusions: MSC-EVs may protect against AKI possibly through anti-oxidation by enhancing Nrf2/ARE activation. (C) 2016 The Author(s) Published by S. Karger AG, Basel
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