Transcriptome analyses revealed chilling response genes in mango (Mangifera indica L. cv. Keitt) leaf

Mangifera indica L. cv. Keitt is a cold-stress fruit plant native to China's drought river valley. Chilling stress affects productivity. Understanding the mechanisms of chilling stress is important to increasing chilling resistance in mango. Leaves of Keitti were subjected to 4 degrees C for 0, 3, 6, and 9 h for RNA-Seq-based transcriptome analysis, respectively. The chlorophyll content, carotenoid content, catalase, and peroxidase activities significantly increased during 9 h. The leaves responded to the stress by enhancing photosynthetic pigment content and antioxidant enzyme activity. After 3 h of chilling, 410 genes were differentially expressed. WRKY70 and PLD1 were significantly up-regulated after 9 h. Compared to 9 and 0 h, there were 1123 DEG. The DEGs are enriched in hormonal signal transduction, secondary metabolites, and the abiotic stress response. Similarly, the transcriptional factor families including NCED2, MYB73, and HLH162 up-regulated. The study will promote research on the development of chill-resistant mangoes.

Automated summary

Transcriptome analysis revealed that Mangifera indica L. cv. Keitt responds to chilling stress by enhancing photosynthetic pigment content and antioxidant enzyme activity. After 3 hours of chilling, 410 genes showed differential expression, with WRKY70 and PLD1 significantly up-regulated after 9 hours. Compared to 9 and 0 hours, there were 1123 differentially expressed genes (DEGs). DEGs were enriched in hormonal signal transduction, secondary metabolites, and the abiotic stress response. This study will contribute to the development of chill-resistant mangoes.