Journal
MBIO
Volume -, Issue -, Pages -Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/mbio.03303-22
Keywords
core protein; hepatitis B virus; live cell imaging; nuclear export; subcellular trafficking; virus assembly
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Hepatitis B virus (HBV) capsid assembly is initially observed in the nucleus and later redistributed to the cytoplasm during nuclear envelope breakdown and cell division. This study provides dynamic evidence supporting the nucleus as an early-stage site of HBV capsid assembly and the cytoplasmic retention of capsids after cell division as a mechanism for nucleus-to-cytoplasm relocalization.
Hepatitis B virus (HBV) capsid assembly is traditionally thought to occur predominantly in the cytoplasm, where the virus gains access to the virion egress pathway. To better define sites of HBV capsid assembly, we carried out single cell imaging of HBV Core protein (Cp) subcellular trafficking over time under conditions supporting genome packaging and reverse transcription in Huh7 hepatocellular carcinoma cells. Time-course analyses including live cell imaging of fluorescently tagged Cp derivatives showed Cp to accumulate in the nucleus at early time points (similar to 24 h), followed by a marked re-distribution to the cytoplasm at 48 to 72 h. Nucleus-associated Cp was confirmed to be capsid and/or high-order assemblages using a novel dual label immunofluorescence strategy. Nuclear-to-cytoplasmic re-localization of Cp occurred predominantly during nuclear envelope breakdown in conjunction with cell division, followed by strong cytoplasmic retention of Cp. Blocking cell division resulted in strong nuclear entrapment of high-order assemblages. A Cp mutant, Cp-V124W, predicted to exhibit enhanced assembly kinetics, also first trafficked to the nucleus to accumulate at nucleoli, consistent with the hypothesis that Cp's transit to the nucleus is a strong and constitutive process. Taken together, these results provide support for the nucleus as an early-stage site of HBV capsid assembly, and provide the first dynamic evidence of cytoplasmic retention after cell division as a mechanism underpinning capsid nucleus-to-cytoplasm relocalization.
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