Journal
BIOANALYSIS
Volume -, Issue -, Pages -Publisher
Newlands Press Ltd
DOI: 10.4155/bio-2023-0018
Keywords
DBS; dried blood spot; hematocrit; hematocrit effect; image analysis; microsampling; nondestructive; therapeutic drug monitoring
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The aim of this study was to develop a nondestructive method to estimate hematocrit in dried blood spots. Standards and controls were created, and the DBS were scanned using a flatbed scanner. Gray values and pixel areas were analyzed with open-source software to estimate hematocrit and volume. The results were compared with hematological analyzer and showed high precision and accuracy.
Aim: To develop a nondestructive method for the estimation of hematocrit (HCT) in dried blood spots (DBSs). Materials & methods: Standards and controls were created (HCT range: 0.20-0.50 l/l) and DBS scanned using a flatbed scanner. Gray values and pixel areas were analyzed with open-source software to estimate HCT and volume, respectively. HCT obtained in whole blood using hematological analyzer was compared with DBS scanner method (n = 50). Results: Between-run precision was 4.7-10.2% and between-run accuracy was 89.6-102.1%. In the hematological instrument comparison, 96% of the patient sample results were within +/- 15%. Conclusion: The nondestructive method can be used to exclude patient DBS samples with extreme HCT levels from further analysis and avoid bias on measured concentration.
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