Journal
VIRUSES-BASEL
Volume 15, Issue 4, Pages -Publisher
MDPI
DOI: 10.3390/v15040959
Keywords
RYMV; rice; resistance; virulence; detection
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Rice yellow mottle virus (RYMV) is a major constraint to rice cultivation in Africa, with high genetic diversity. This study developed specific RT-PCR primers to infer the resistance-breaking abilities of RYMV isolates, providing a molecular tool for optimizing the deployment strategy of resistant rice lines.
Rice yellow mottle virus (RYMV) is a major biotic constraint to rice cultivation in Africa. RYMV shows a high genetic diversity. Viral lineages were defined according to the coat protein (CP) phylogeny. Varietal selection is considered as the most efficient way to manage RYMV. Sources of high resistance were identified mostly in accessions of the African rice species, Oryza glaberrima. Emergence of resistance-breaking (RB) genotypes was observed in controlled conditions. The RB ability was highly contrasted, depending on the resistance sources and on the RYMV lineages. A molecular marker linked to the adaptation to susceptible and resistant O. glaberrima was identified in the viral protein genome-linked (VPg). By contrast, as no molecular method was available to identify the hypervirulent lineage able to overcome all known resistance sources, plant inoculation assays were still required. Here, we designed specific RT-PCR primers to infer the RB abilities of RYMV isolates without greenhouse experiments or sequencing steps. These primers were tested and validated on 52 isolates, representative of RYMV genetic diversity. The molecular tools described in this study will contribute to optimizing the deployment strategy of resistant lines, considering the RYMV lineages identified in fields and their potential adaptability.
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