A Rice Receptor-like Protein Negatively Regulates Rice Resistance to Southern Rice Black-Streaked Dwarf Virus Infection

Plants rely on various receptor-like proteins and receptor-like kinases to recognize and defend against invading pathogens. However, research on the role of receptor-like proteins in plant antiviral defense, particularly in rice-virus interactions, is limited. In this study, we identified a receptor-like gene, OsBAP1, which was significantly induced upon infection with southern rice black-streaked dwarf virus (SRBSDV) infection. A viral inoculation assay showed that the OsBAP1 knockout mutant exhibited enhanced resistance to SRBSDV infection, indicating that OsBAP1 plays a negatively regulated role in rice resistance to viral infection. Transcriptome analysis revealed that the genes involved in plant-pathogen interactions, plant hormone signal transduction, oxidation-reduction reactions, and protein phosphorylation pathways were significantly enriched in OsBAP1 mutant plants (osbap1-cas). Quantitative real-time PCR (RT-qPCR) analysis further demonstrated that some defense-related genes were significantly induced during SRBSDV infection in osbap1-cas mutants. Our findings provide new insights into the role of receptor-like proteins in plant immune signaling pathways, and demonstrate that OsBAP1 negatively regulates rice resistance to SRBSDV infection.

Automated summary

Plants utilize receptor-like proteins and receptor-like kinases to identify and defend against invading pathogens. However, the role of receptor-like proteins in plant antiviral defense, specifically in rice-virus interactions, remains limited. This study identified a receptor-like gene, OsBAP1, which showed an increased expression upon infection with southern rice black-streaked dwarf virus (SRBSDV). Knockout mutant analysis demonstrated that OsBAP1 negatively regulates rice resistance to SRBSDV infection. Transcriptome analysis revealed that defense-related genes were significantly enriched in OsBAP1 mutant plants, further supporting its role in plant immunity pathways.