Journal
VIROLOGY
Volume 583, Issue -, Pages 1-13Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2023.03.016
Keywords
Dengue virus; NS5; STAT2; Apigenin; Luteolin; Cell-based assays
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Dengue virus (DENV) evades the immune response by degrading STAT2 using the NS5 protein. The compounds Apigenin and Luteolin inhibit DENV replication and restore STAT2 phosphorylation even in the absence of infection. This highlights the importance of screening compounds that can interact with host factors to counteract viral proteins that dampen innate immune responses.
Type I interferon (IFN-I) evasion by Dengue virus (DENV) is key in DENV pathogenesis. The non-structural protein 5 (NS5) antagonizes IFN-I response through the degradation of the signal transducer and activator of transcription 2 (STAT2). We developed a K562 cell-based platform, for high throughput screening of compounds potentially counteracting the NS5-mediated antagonism of IFN-I signaling. Upon a screening with a library of 1220 approved drugs, 3 compounds previously linked to DENV inhibition (Apigenin, Chrysin, and Luteolin) were identified. Luteolin and Apigenin determined a significant inhibition of DENV2 replication in Huh7 cells and the restoration of STAT2 phosphorylation in both cell systems. Apigenin and Luteolin were able to stimulate STAT2 even in the absence of infection. Despite the promiscuous and pan-assay-interfering nature of Luteolin, Apigenin promotes STAT2 Tyr 689 phosphorylation and activation, highlighting the importance of screening for compounds able to interact with host factors, to counteract viral proteins capable of dampening innate immune responses.
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