Dengue Virus Increases the Expression of TREM-1 and CD10 on Human Neutrophils

Every year, dengue is responsible for 400 million infections worldwide. Inflammation is related to the development of severe forms of dengue. Neutrophils are a heterogeneous cell population with a key role in the immune response. During viral infection, neutrophils are mainly recruited to the infection site; however, their excessive activation is linked to deleterious results. During dengue infection, neutrophils are involved in the pathogenesis through neutrophils extracellular traps production, tumor necrosis factor-alpha, and interleukin-8 secretion. However, other molecules regulate the neutrophil role during viral infection. TREM-1 is expressed on neutrophils and its activation is related to increased production of inflammatory mediators. CD10 is expressed on mature neutrophils and has been associated with the regulation of neutrophil migration and immunosuppression. However, the role of both molecules during viral infection is limited, particularly during dengue infection. Here, we report for the first time that DENV-2 can significantly increase TREM-1 and CD10 expression as well as sTREM-1 production in cultured human neutrophils. Furthermore, we observed that treatment with granulocyte-macrophage colony stimulating factor, a molecule mostly produced in severe cases of dengue, is capable of inducing the overexpression of TREM-1 and CD10 on human neutrophils. These results suggest the participation of neutrophil CD10 and TREM-1 in the pathogenesis of dengue infection.

Automated summary

Dengue is a major global health problem, with 400 million infections reported annually. Neutrophils play a crucial role in the immune response during dengue infection. Their activation can lead to detrimental effects, while their involvement in the pathogenesis is regulated by various molecules. This study found that the expression of TREM-1 and CD10, as well as the production of sTREM-1, were significantly increased in human neutrophils infected with DENV-2 or treated with granulocyte-macrophage colony stimulating factor. These findings suggest that neutrophil CD10 and TREM-1 are involved in the pathogenesis of dengue infection.