Detection and genetic characterization of circoviruses in more than 80 bat species from eight countries on four continents

Several bat-associated circoviruses and circular rep-encoding single-stranded DNA (CRESS DNA) viruses have been described, but the exact diversity and host species of these viruses are often unknown. Our goal was to describe the diversity of bat-associated circoviruses and cirliviruses, thus, 424 bat samples from more than 80 species were collected on four continents. The samples were screened for circoviruses using PCR and the resulting amino acid sequences were subjected to phylogenetic analysis. The majority of bat strains were classified in the genus Circovirus and some strains in the genus Cyclovirus and the clades CRESS1 and CRESS3. Some strains, however, could only be classified at the taxonomic level of the order and were not classified in any of the accepted or proposed clades. In the family Circoviridae, 71 new species have been predicted. This screening of bat samples revealed a great diversity of circoviruses and cirliviruses. These studies underline the importance of the discovery and description of new cirliviruses and the need to establish new species and families in the order Cirlivirales.

Automated summary

By screening and analyzing 424 bat samples from more than 80 species on four continents, a wide diversity of bat-associated circoviruses and circular rep-encoding single-stranded DNA (CRESS DNA) viruses were identified. Most of the bat strains belonged to the genus Circovirus, while some belonged to the genus Cyclovirus and clades CRESS1 and CRESS3. Additionally, 71 new species were predicted in the family Circoviridae. These findings highlight the importance of discovering and describing new cirliviruses and the establishment of new species and families in the order Cirlivirales.