Iris angiography in ADAMTS10 mutant dogs with open-angle glaucoma (ADAMTS10-OAG)

ObjectiveTo evaluate anterior segment angiographic findings in hypertensive ADAMTS10-open-angle glaucoma (ADAMTS10-OAG) eyes as compared to normotensive control eyes. Animals StudiedNine ADAMTS10-OAG beagles and four wild-type control dogs. ProceduresAnterior segment angiography was performed under general anesthesia following intravenous injection of indocyanine green (ICG; 1 mg/kg) and sodium fluorescein (SF; 20 mg/kg) using a Heidelberg Spectralis (R) confocal scanning laser ophthalmoscope. Time to onset of iridal angiographic phases and the presence/severity of dye leakage into the iris stromal and/or aqueous humor were recorded. Group findings were compared, and multiple linear regression analysis was performed to identify potential factor associations with disease status. ResultsTime to onset of all angiographic phases visualized using ICG was significantly prolonged while time to onset of SF leakage into the aqueous humor was significantly reduced in glaucomatous eyes compared to controls. Only glaucomatous eyes (n = 9) demonstrated evidence of SF stromal leakage. Mean intraocular pressure (IOP) and age were significantly higher, while mean cardiac pulse was significantly lower in glaucomatous eyes compared to controls. Blood pressure and ocular perfusion pressure were not significantly different between groups. Multiple linear regression analysis, controlling for age, IOP, and pulse demonstrated glaucoma, was not predictive of the time to onset of any angiographic phase, stromal, or aqueous humor leakage. However, pulse was a significant factor contributing to the severity of aqueous humor leakage. ConclusionsA compromised vascular supply to the anterior segment exists in dogs with ADAMTS10-OAG. These observations warrant further exploration of what role altered perfusion and/or disruption to the blood-aqueous barrier may play.

Automated summary

This study aimed to evaluate the anterior segment angiographic findings in hypertensive ADAMTS10-open-angle glaucoma (ADAMTS10-OAG) eyes compared to normotensive control eyes. The results showed that the time to onset of all angiographic phases was significantly prolonged, while the time to onset of sodium fluorescein (SF) leakage into the aqueous humor was significantly reduced in glaucomatous eyes. Only glaucomatous eyes demonstrated evidence of SF stromal leakage. Multiple linear regression analysis indicated that glaucoma was not predictive of the time to onset of any angiographic phase or leakage, but pulse was a significant factor contributing to the severity of aqueous humor leakage.