Journal
JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
Volume 17, Issue 6, Pages 425-436Publisher
ZHEJIANG UNIV
DOI: 10.1631/jzus.B1500213
Keywords
Gardenblue blueberry (Vaccinium ashei Reade) anthocyanin extracts (GBBAEs); Anti-inflammatory; RAW 264.7; Cyclooxygenase 2 (COX-2); Nuclear factor-kappa B p65 (NF-kappa Bp65)
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Funding
- Fundamental Research Funds for the Central Universities of China [2013PY095]
- Clinical Research Project of Health and Family Planning Commission of Wuhan Municipality [WX13A05]
- Research Project of Wuhan City Central Hospital [YQ15A04]
- Key Laboratory of Biological Targeted Therapy of Hubei Province of China [02.03.2014-10]
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Blueberries are a rich source of anthocyanins, which are associated with health benefits contributing to a reduced risk for many diseases. The present study identified the functional Gardenblue blueberry (Vaccinium ashei Reade) anthocyanin extracts (GBBAEs) and evaluated their capacity and underlying mechanisms in protecting murine RAW 264.7 cells from lipopolysaccharide (LPS)-stimulated inflammation in vitro. Enzyme-linked immunosorbent assay (ELISA) kit results showed that GBBAEs significantly inhibited the production of nitric oxide (NO), prostaglandin E-2 (PGE(2)), interleukin-6 (IL-6), IL-1 beta, and interferon-gamma (INF-gamma). Real-time polymerase chain reaction (PCR) analysis indicated that the mRNA expression levels of IL-6, IL-1 beta, tumor necrosis factor-alpha (TNF-alpha), monocyte chemoattractant protein-1 (MCP-1), and cyclooxygenase 2 (COX-2) were suppressed in LPS-stimulated RAW 264.7 cells. Additionally, Western blot analysis was used to evaluate the relative protein expression levels of COX-2 and nuclear factor-kappa B p65 (NF-kappa Bp65). All these results suggested the potential use of GBBAEs as a functional food for the treatment of inflammatory diseases.
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