Journal
TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE
Volume 117, Issue 9, Pages 628-636Publisher
OXFORD UNIV PRESS
DOI: 10.1093/trstmh/trad016
Keywords
anti-HBs Ab; Hepatitis B vaccine; memory B cells
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This study aimed to evaluate and compare the frequency of various B cell subpopulations in responders and non-responders to HBsAg vaccine. The results found that the memory B cell populations were comparable between the responder and non-responder groups. Further investigation is needed to determine if the level of class switching in B lymphocytes is correlated with anti-HBsAb production in healthy vaccinated individuals.
Background Hepatitis B is a major global health problem. More than 90% of hepatitis B-vaccinated immunocompetent adults become fully immune. The main purpose of vaccination is immunization. Whether non-responders have a lower percentage of total or antigen-specific memory B cells in comparison with responders is still controversial. We aimed to assess and compare the frequency of various B cell subpopulations in non-responders and responders.Methods Fourteen responders and 14 non-responders of hospital healthcare workers were enrolled in this study. We used flow cytometry to evaluate various CD19(+) B cell subpopulations using fluorescent-labeled antibodies against CD19, CD10, CD21, CD27 and IgM and ELISA to evaluate total anti-HBs antibodies.Results We found no significant differences in the frequency of various B cell subpopulations between the non-responder and responder groups. Furthermore, the frequency of the isotype-switched memory B cell population was significantly higher in the atypical memory B cell subset compared with the classical memory B cell subset in the responder and total groups (p=0.010 and 0.003, respectively).Conclusions Responders and non-responders to HBsAg vaccine had comparable memory B cell populations. Whether anti-HBs Ab production has a correlation with the level of class switching in B lymphocytes in healthy vaccinated individuals needs further investigation.
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