Journal
TOXICON
Volume 226, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.toxicon.2023.107081
Keywords
Tetrodotoxin; Monoclonal antibody; 5; 6; 11-trideoxyTetrodotoxin; 11-norTetrodotoxin-6(S)-ol; 11-oxoTetrodotoxin; ELISA
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This study confirmed the low cross-reactivity of a monoclonal antibody against tetrodotoxin (TTX) to three major TTX analogues in pufferfish using competitive ELISA. The reactivity against TTX was 100%, while the cross-reactivity to 5,6,11-trideoxyTTX, 11-norTTX-6(S)-ol, and 11-oxoTTX was less than 2.2%, 0.3%, and 1.5% respectively. Additionally, the presence of these analogues did not cause overestimation of TTX in pufferfish extracts using competitive ELISA.
The monoclonal antibody against tetrodotoxin (TTX), prepared by Kawatsu et al. (1997), has been used in several TTX-related studies. Herein, we confirmed the quite low cross-reactivity of this antibody to three major TTX analogues in pufferfish using competitive ELISA: 5,6,11-trideoxyTTX (<2.2%), 11-norTTX-6(S)-ol (<0.3%), and 11-oxoTTX (<1.5%), with reactivity against TTX being 100%. We further confirmed that the presence of these analogues did not cause a marked overestimation of TTX in pufferfish extracts using competitive ELISA.
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