Journal
TALANTA
Volume 258, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2023.124389
Keywords
Untargeted metabolomics; Ion mobility; Chemical signalling; Mouse pheromones; Maternal care; HRMS
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This study aimed to determine the low-volatile chemosignals excreted or secreted by mouse pups in their early days of life, which are involved in maternal care induction in adult female mice. Untargeted metabolomics was used to differentiate samples collected from the facial and anogenital area of neonatal mouse pups receiving maternal care and elder mouse pups in the weaning period. The results showed the potential of UHPLC-IMS-HRMS based untargeted metabolomics in identifying putative pheromones in mammals.
The present study is focused on the determination of low-volatile chemosignals excreted or secreted by mouse pups in their early days of life involved in maternal care induction in mice adult females. Untargeted metab-olomics was employed to differentiate between samples collected with swabs from facial and anogenital area from neonatal mouse pups receiving maternal care (first two weeks of life) and the elder mouse pups in the weaning period (4th week old). The sample extracts were analysed by ultra-high pressure liquid chromatography (UHPLC) coupled to ion mobility separation (IMS) in combination with high resolution mass spectrometry (HRMS). After data processing with Progenesis QI and multivariate statistical analysis, five markers present in the first two weeks of mouse pups life and putatively involved in materno-filial chemical communication were tentatively identified: arginine, urocanic acid, erythro-sphingosine (d17:1), sphingosine (d18:1) and sphinga-nine. The four-dimensional data and the tools associated to the additional structural descriptor obtained by IMS separation were of great help in the compound identification. The results demonstrated the great potential of UHPLC-IMS-HRMS based untargeted metabolomics to identity putative pheromones in mammals.
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