Application of aminobenzoic acid electrodeposited screen-printed carbon electrode in the beta-amyloid electrochemical impedance spectroscopy immunoassay

Alzheimer's disease (AD) is one of the important neurodegenerative diseases, in the modern aging society, it has become an issue people need to work on. Of the pathogenic factor which leads to AD, beta-amyloid (A ss) is the most important one. It can form the senile plaque which aggregates in the neuron and interrupts the signal transmission. This research is based on the electrochemical system and screen-printed carbon electrode (SPCE) incorporated with pretreatment, electrodeposition, electrochemical impedance spectroscopy (EIS), antibody, and blocking agent. This immunosensor is applied to detect the different concentrations of A ss. The standard curve between electrical impedance and concentration of A ss is calculated. The specificity of the immunosensor is tested. This survey optimizes the electrodeposition condition for 4-aminobenzoic acid (4-ABA) and the parameter for antibody and blocking agents. This study fabricates a more dense, uniform, and stable film of 4-ABA. This sensor presents a range of detection from 1 fg/ml to 100 pg/ml and a limit of detection to 3.84 fg/ml. This sensor can identify the isoform of A ss. This research shortens the fabricating time to 3.5 h. This study fabricates a labelfree and low-cost immunosensor for A ss with a short fabricating time, high stability, wide range of detection, low limit of detection, and good specificity. The impedance of the carbon printed electrodes is very high and is always measured by its current but this study provides a fabrication technique for high-efficiency carbon printed electrodes for electrochemical impedance spectroscopy sensing.

Automated summary

Alzheimer's disease is an important neurodegenerative disease in the aging society, with beta-amyloid being a crucial factor in its development. This research focuses on developing an immunosensor using an electrochemical system and screen-printed carbon electrode to detect beta-amyloid. The study optimizes the electrodeposition conditions and tests the sensor's specificity, achieving a labelfree and low-cost immunosensor with high stability and a wide range of detection.