Feasible spectrofluorimetric approach for the ultrasensitive determination of lomefloxacin based on synergistic effects of micellization and metal complexation

The purpose of the present work was to establish a fast and convenient strategy for lomefloxacin analysis using a fluorimetric approach. The methodology was based on the complex formation of the drug with aluminum ion to give a product having high fluorescence. Adding sodium dodecyl sulfate led to further boosting the intensity of fluorescence which was recorded at 429 nm after excitation at 332 nm. The relationship of emission intensity with lomefloxacin concentration was linear at 10-130 ng mL-1 with a correlation coefficient of 0.9996. The quantitation limit was 11.4 ng mL-1 and detection limit was 3.8 ng mL-1. The reaction conditions were carefully studied which included the pH, buffer type, its concentration, the type and concentration of surfactant and the diluting solvent. The method was utilized to quantify the aforementioned drug in tablet formulations and in real human plasma with high accuracy and reliability.

Automated summary

The aim of this study was to develop a rapid and convenient fluorimetric method for analyzing lomefloxacin. The method utilized the complex formation between the drug and aluminum ion to produce a highly fluorescent product. The addition of sodium dodecyl sulfate further enhanced the fluorescence intensity, which was measured at 429 nm after excitation at 332 nm. The relationship between emission intensity and lomefloxacin concentration was linear from 10-130 ng mL-1, with a correlation coefficient of 0.9996. The method demonstrated high accuracy and reliability in quantifying the drug in tablet formulations and real human plasma.