Raman imaging monitors the time-resolved response of A. thaliana to the artificial inhibition of PSII

The short-term (0-96 h) response of A. thaliana to the oxidative stress induced by PSII inhibitor metribuzin was examined using Raman spectroscopy. Whole leaves of wildtype (WT, Col-0) and ros1 mutant were scanned and changes in carotenoids were examined. Strong differences in Raman intensity distributions between WT and ros1 were observed. A stronger decrease of carotenoid v1(C=C) band intensity across the leaf was observed in ros1 after 48 h of exposure to metribuzin. It can be assumed that higher sensitivity to oxidative stress in ros1 mutant results in significantly faster degradation of carotenoids.

Automated summary

Raman spectroscopy was used to investigate the short-term (0-96 h) response of A. thaliana to oxidative stress induced by the PSII inhibitor metribuzin. Whole leaves of wildtype (WT, Col-0) and ros1 mutant were scanned, and changes in carotenoids were examined. Significant differences in Raman intensity distributions between WT and ros1 were observed, with a stronger decrease in carotenoid v1(C=C) band intensity across the leaf in ros1 after 48 h of metribuzin exposure.