Journal
SMALL
Volume 19, Issue 23, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.202206160
Keywords
death receptors; DNA origami; interligand distance; nanoscale organization; TRAIL trimers; tumor therapy
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In this study, a flat rectangular DNA origami is used as a display scaffold and a strategy called engraving-printing is developed to rapidly decorate three TRAIL monomers onto its surface to form DNA-TRAIL3 trimers. By comparing the receptor affinity, agonistic activity, and cytotoxicity, it is found that the critical interligand distance of DNA-TRAIL3 trimers to induce death receptor clustering and resulting apoptosis is approximately 40 nm.
Through inducing death receptor (DR) clustering to activate downstream signaling, tumor necrosis factor related apoptosis inducing ligand (TRAIL) trimers trigger apoptosis of tumor cells. However, the poor agonistic activity of current TRAIL-based therapeutics limits their antitumor efficiency. The nanoscale spatial organization of TRAIL trimers at different interligand distances is still challenging, which is essential for the understanding of interaction pattern between TRAIL and DR. In this study, a flat rectangular DNA origami is employed as display scaffold, and an engraving-printing strategy is developed to rapidly decorate three TRAIL monomers onto its surface to form DNA-TRAIL3 trimer (DNA origami with surface decoration of three TRAIL monomers). With the spatial addressability of DNA origami, the interligand distances are precisely controlled from 15 to 60 nm. Through comparing the receptor affinity, agonistic activity and cytotoxicity of these DNA-TRAIL3 trimers, it is found that approximate to 40 nm is the critical interligand distance of DNA-TRAIL3 trimers to induce death receptor clustering and the resulting apoptosis.Finally, a hypothetical active unit model is proposed for the DR5 clustering induced by DNA-TRAIL3 trimers.
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