Journal
SEPARATION AND PURIFICATION TECHNOLOGY
Volume 308, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.seppur.2022.122941
Keywords
Molecularly imprinted membrane; Selectivity; Lysozyme; Mesoporous silica nanoparticles; Double-faced self -assembly
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The combination of membranes and nanomaterials has made significant progress in protein separation. However, current methods suffer from aggregation and embedding of nanomaterials, affecting membrane performance. In this study, lysozyme molecularly imprinted nanocomposite membranes (LYZ-MINMs) with carboxyl functionalized mesoporous silica nanoparticles (C-MSNs) layer were successfully prepared for selective separation of lysozyme from chicken egg white. LYZ-MINMs showed improved hydrophilicity and rebinding capacity compared to other methods. The high selectivity and regeneration rate of LYZ-MINMs demonstrate their potential for practical applications in protein separation.
In recent years, the combination of membranes and nanomaterials has made remarkable progress in separation of proteins. Nevertheless, the current combination methods suffer from the drawbacks of aggregation and embedding of nanomaterials, leading to a loss of membrane performance. In present work, the lysozyme molecularly imprinted nanocomposite membranes (LYZ-MINMs) with uniform and dense carboxyl functionalized mesoporous silica nanoparticles (C-MSNs) layer were successfully prepared by a double-faced self-assembly strategy for selective separation of LYZ from chicken egg white. Notably, the resulted LYZ-MINMs exhibit both promoted hydrophilicity (36.2 degrees) and enhanced rebinding capacity (509 mg g-1) by comparing with the MINMs designed with the blending method. The selectivity of LYZ-MINMs toward LYZ is improved by the sol-gel imprinting process, resulting in a satisfactory rebinding selectivity of 2.98, 3.22 and 3.45 for Cytochrome C , Bovine haemoglobin, and Ovalbumin, respectively. After ten cycles, the high regeneration rate (>90 %) dem-onstrates the potential of LYZ-MINMs for practical applications. In addition, the practicability of LYZ-MINMs further verified by utilizing diluted egg white. The work shows great promise for next-generation membranes for protein separation.
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