Development of a Disposable Polyacrylamide Hydrogel-Based Semipermeable Membrane for Micro Ag/AgCl Reference Electrode

Currently, Ag/AgCl-based reference electrodes are used in most electrochemical biosensors and other bioelectrochemical devices. However, standard reference electrodes are rather large and do not always fit within electrochemical cells designed for the determination of analytes in low-volume aliquots. Therefore, various designs and improvements in reference electrodes are critical for the future development of electrochemical biosensors and other bioelectrochemical devices. In this study, we explain a procedure to apply common laboratory polyacrylamide hydrogel in a semipermeable junction membrane between the Ag/AgCl reference electrode and the electrochemical cell. During this research, we have created disposable, easily scalable, and reproducible membranes suitable for the design of reference electrodes. Thus, we came up with castable semipermeable membranes for reference electrodes. Performed experiments highlighted the most suitable gel formation conditions to achieve optimal porosity. Here, Cl- ion diffusion through the designed polymeric junctions was evaluated. The designed reference electrode was also tested in a three-electrode flow system. The results show that home-built electrodes can compete with commercial products due to low reference electrode potential deviation (similar to 3 mV), long shelf-life (up to six months), good stability, low cost, and disposability. The results show a high response rate, which makes in-house formed polyacrylamide gel junctions good membrane alternatives in the design of reference electrodes, especially for these applications where high-intensity dyes or toxic compounds are used and therefore disposable electrodes are required.

Automated summary

Ag/AgCl-based reference electrodes are widely used in electrochemical biosensors and bioelectrochemical devices. However, the large size of standard reference electrodes poses challenges when working with low-volume samples. This study presents a procedure to incorporate polyacrylamide hydrogel as a semipermeable junction membrane in Ag/AgCl reference electrodes, creating disposable, reproducible, and scalable membranes suitable for design. Experimental results demonstrate the efficacy of the home-built electrodes, which offer low potential deviation, long shelf-life, good stability, low cost, disposability, and high response rate, making them suitable for applications involving intense dyes or toxic compounds.