Paeoniflorin attenuates polystyrene nanoparticle-induced reduction in reproductive capacity and increase in germline apoptosis through suppressing DNA damage checkpoints in Caenorhabditis elegans

Due to high sensitivity to environmental exposures, Caenorhabditis elegans is helpful for toxicity evaluation and toxico-logical study of pollutants. Using this animal model, we investigated the reproductive toxicity of 20 nm polystyrene nanoparticle (PS-NP) in the range of mu g/L and the following pharmacological intervention of paeoniflorin. After expo-sure from L1-larvae to young adults, 10-100 mu g/L PS-NP could cause the reduction in reproductive capacity reflected by the endpoints of brood size and number of fertilized eggs in uterus. Meanwhile, the enhancements in germline ap-optosis analyzed by AO staining and germline DNA damage as shown by alteration in HUS-1::GFP signals were de-tected in 10-100 mu g/L PS-NP exposed nematodes, suggesting the role of DNA damage-induced germline apoptosis in mediating PS-NP toxicity on reproductive capacity. Following the exposure to 100 mu g/L PS-NP, posttreatment with 25-100 mg/L paeoniflorin increased the reproductive capacity and inhibited both germline apoptosis and DNA damage. In addition, in 100 mu g/L PS-NP exposed nematodes, treatment with 100 mg/L paeoniflorin modulated the expressions of genes governing germline apoptosis as indicated by the decrease in ced-3, ced-4, an egl-1 expressions and the increase in ced-9 expression. After exposure to 100 mu g/L PS-NP, treatment with 100 mg/L paeoniflorin also de-creased expressions of genes (cep-1, clk-2, hus-1, and mrt-2) governing germline DNA damage. Molecular docking anal-ysis further demonstrated the binding potential of paeoniflorin with three DNA damage checkpoints (CLK-2, HUS-1, and MRT-2). Therefore, our data suggested the toxicity of PS-NP in the range of mu g/L on reproductive capacity after exposure from L1-larvae to young adults, which was associated with the enhancement in DNA damage-induced germ-line apoptosis. More importantly, the PS-NP-induced reproductive toxicity on nematodes could be inhibited by the fol-lowing paeoniflorin treatment.

Automated summary

The reproductive toxicity of 20 nm polystyrene nanoparticles (PS-NP) in Caenorhabditis elegans was investigated, and it was found that PS-NP exposure reduced reproductive capacity and induced DNA damage-induced germline apoptosis. Treatment with paeoniflorin had a protective effect on reproductive capacity and reduced germline apoptosis and DNA damage.