4.7 Article

Genome mining-guided activation of two silenced tandem genes in Raoultella ornithinolytica XF201 for complete biodegradation of phthalate acid esters

Journal

SCIENCE OF THE TOTAL ENVIRONMENT
Volume 864, Issue -, Pages -

Publisher

ELSEVIER
DOI: 10.1016/j.scitotenv.2022.161013

Keywords

Emerging contaminants; Phthalate acid esters; Genome mining; Ribosome engineering; Bioremediation

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Genome mining identified bacteria with potential to degrade phthalates (PAEs), which are widely present in soils and food products and pose a high risk to human health. Ribosome engineering was used to activate the expression of key genes involved in PAEs biodegradation, resulting in a mutant strain XF201-G2U5 that showed high activity in degrading di-n butyl phthalate (DBP) and other PAEs. This strategy enables the discovery of novel bacterial resources for the bioremediation of PAEs and other emerging contaminants.
Phthalates (PAEs) are ubiquitous in soils and food products and thus pose a high risk to human health. Herein, genome mining revealed a great diversity of bacteria with PAEs-degrading potential. Mining of the genome of Raoultella ornithinolytica XF201, a novel strain isolated from Dongxiang wild rice rhizosphere, revealed the presence of two si-lenced tandem genes pcdGH (encoding protocatechuate 3,4-dioxygenase, 3,4-PCD), key aromatic ring-cleaving genes in PAEs biodegradation. Ribosome engineering was successfully utilized to activate the expression of pcdGH genes to produce 3,4-PCD in the mutant XF201-G2U5. The mutant XF201-G2U5 showed high 3,4-PCD activity and could remove 94.5 % of di-n butyl phthalate (DBP) in 72 h. The degradation kinetics obeyed the first-order kinetic model. Strain XF201-G2U5 could also degrade the other PAEs and the main intermediate metabolites, ultimately lead-ing to tricarboxylic acid cycle. Therefore, this strategy facilitates novel bacterial resources discovery for bioremedia-tion of PAEs and other emerging contaminants.

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