Long-term exposure to a pharmaceutical pollutant affects geotaxic behaviour in the adult but not juvenile life stage of killifish

Ecosystems around the world are increasingly polluted with pharmaceutical compounds that may perturb wildlife behaviour. Because many pharmaceuticals are continuously present in the aquatic environment, animals are often ex-posed to them across several life stages or even their entire life. Despite a large body of literature showing various im-pacts of exposure to pharmaceuticals on fish, hardly any long-term studies across different life stages have been conducted which makes it hard to accurately estimate the ecological outcomes of pharmaceutical pollution. Here, we performed a laboratory experiment in which we exposed hatchlings of the fish model Nothobranchius furzeri to an environmentally relevant concentration (0.5 mu g/L) of the antidepressant fluoxetine until well into adulthood. We monitored total body length and geotaxic behaviour (i.e. gravity-mediated activity) of each fish as two traits that are ecologically relevant and naturally differ between juvenile and adult killifish. Fish exposed to fluoxetine were smaller compared to control fish, an effect that became more apparent as fish aged. Even though fluoxetine did not affect average swimming depth of either juveniles or adults, nor the time spent at the surface or bottom of the water column, exposed fish changed their position in the water column (depth) more frequently in the adult but not juvenile phase. These results suggest that important morphological and behavioural responses to pharmaceutical exposure-and their potential ecological consequences-may only emerge later in time and/or during specific life stages. Therefore, our results highlight the importance of considering ecologically relevant timescales across developmental stages when studying the ecotoxicology of pharmaceuticals.

Automated summary

Ecosystems worldwide are being polluted by pharmaceutical compounds, which can disrupt wildlife behaviour. Despite a large body of literature, there is a lack of long-term studies across different life stages to accurately estimate the ecological outcomes of pharmaceutical pollution. In this experiment, fish exposed to the antidepressant fluoxetine showed smaller body size and changes in their position in the water column during adulthood, highlighting the importance of considering ecologically relevant timescales when studying the ecotoxicology of pharmaceuticals.