Advances in bacterial whole-cell biosensors for the detection of bioavailable mercury: A review

Mercury (Hg) and its organic compounds, especially monomethylmercury (MeHg), cause major damage to the ecosystem and human health. In surface water or sediments, microorganisms play a crucial role in the methylation and demethylation of Hg. Given that Hg transformation processes are intracellular reactions, accurate assessment of the bioavailability of Hg(II)/MeHg in the environment, particularly for microorganisms, is of major importance. Compared with traditional analytical methods, bacterial whole-cell biosensors (BWCBs) provide a more accurate, convenient, and cost-effective strategy to assess the environmental risks of Hg(II)/MeHg. This Review summarizes recent progress in the application of BWCBs in the detection of bioavailable Hg(II)/MeHg, providing insight on current challenges and strategies. The principle and components of BWCBs for Hg(II)/MeHg bioavailability analysis are introduced. Furthermore, the impact of water chemical factors on the bioavailability of Hg is discussed as are future perspectives of BWCBs in bioavailable Hg analysis and optimization of BWCBs.

Automated summary

Mercury and its organic compounds pose significant threats to the environment and human health. Microorganisms play a critical role in the methylation and demethylation of mercury in water and sediments. Bacterial whole-cell biosensors (BWCBs) offer a more accurate and cost-effective approach to assess the bioavailability of mercury compared to traditional methods. This Review provides an overview of the recent advancements in the application of BWCBs for detecting bioavailable mercury and discusses challenges, strategies, and future prospects.