Combined effects of zinc oxide nanoparticles and melatonin on wheat growth, chlorophyll contents, cadmium (Cd) and zinc uptake under Cd stress

Zinc oxide nanoparticles (ZnONPs) and melatonin (MT) have been known to regulate heavy metal toxicities in plants in some studies, the effect of their combined use on cadmium (Cd) uptake by wheat (Triticum aestivum L.) and under-lying mechanisms is largely unknown. Thus, plant growth, uptake and translocation of Cd mediated by soil applied ZnONPs and foliar applied MT were investigated in wheat grown in Cd polluted soil under ambient conditions. The results depicted that ZnONPs stimulated the growth, chlorophyll contents, and yield of wheat in a dose additive way and this effect was further increased with foliar application of MT. 100 mg/kg of ZnONPs alone enhanced the grain yield by 60.5 % and this increase was about 177.5 % under combined ZnONPs and 100 mu M MT treatment. ZnONPs treatments decreased Cd concentration whereas increased zinc (Zn) concentrations in shoots, roots, husks and grains and the effect was further increased with exogenous MT combined with NPs in a dose-additive way. 50 and 100 mg/kg ZnONPs treatments alone decreased grain Cd by 6.5 %, and 20 % and increased the Zn concentration by 20.1 % and 24 % than control. 100 mg/kg ZnONPs +100 mu M MT treatment decreased the grain Cd by 63.5 % and increased grain Zn by 51 % than control treatment. Total Cd uptake (tissues biomass x Cd concentration in respective tissues) in shoots, roots, husks and grains increased with ZnONPs alone or combined with MT than control whereas soil post-harvest bioavailable Cd concentration decreased with treatments than control. The Cd reduction in grains was due to increase in biomass and Zn concentration thereby decreasing bioavailable Cd in soil and its accumulation in plants. This study suggested that combined use of ZnONPs and MT may provide new approaches for minimizing Cd and biofortification of Zn in edible parts of plants.

Automated summary

This study investigated the effects of zinc oxide nanoparticles (ZnONPs) and melatonin (MT) on the uptake of cadmium (Cd) by wheat. The results showed that ZnONPs stimulated wheat growth and yield, and this effect was enhanced by foliar application of MT. ZnONPs decreased Cd concentration and increased zinc (Zn) concentration in the plant tissues, leading to a decrease in bioavailable Cd in the soil. The combined use of ZnONPs and MT may provide new approaches for reducing Cd uptake and increasing zinc biofortification in edible parts of plants.