Journal
SCIENCE CHINA-LIFE SCIENCES
Volume 66, Issue 9, Pages 2006-2019Publisher
SCIENCE PRESS
DOI: 10.1007/s11427-022-2298-1
Keywords
nutritional iron; goblet cell; gut microbiota; mucin2
Categories
Ask authors/readers for more resources
Iron is an important micronutrient that plays a vital role in host defenses and bacterial pathogenicity. Adequate dietary iron intake improves mucus layer function and decelerates the invasion of pathogenic bacteria.
Iron is an important micronutrient that plays a vital role in host defenses and bacterial pathogenicity. As iron treatments increase the risk of infection by stimulating the growth and virulence of bacterial pathogens, their roles in anti-infection immunity have frequently been underestimated. To estimate whether adequate dietary iron intake would help defend against pathogenic bacterial infection, mice were fed iron-deficient (2 mg kg(-1) feed), iron-sufficient (35 mg kg(-1) feed), or iron-enriched diet (350 mg kg(-1) feed) for 12 weeks, followed by oral infection with Salmonella typhimurium. Our results revealed that dietary iron intake improved mucus layer function and decelerated the invasion of the pathogenic bacteria, Salmonella typhimurium. Positive correlations between serum iron and the number of goblet cells and mucin2 were found in response to total iron intake in mice. Unabsorbed iron in the intestinal tract affected the gut microbiota composition, and the abundance of Bacteroidales, family Muribaculaceae, was positively correlated with their mucin2 expression. However, the results from antibiotic-treated mice showed that the dietary iron-regulated mucin layer function was not microbial-dependent. Furthermore, in vitro studies revealed that ferric citrate directly induced mucin2 expression and promoted the proliferation of goblet cells in both ileal and colonic organoids. Thus, dietary iron intake improves serum iron levels, regulates goblet cell regeneration and mucin layer function, and plays a positive role in the prevention of pathogenic bacteria.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available