4.7 Article

Effect of type I interferon on engineered pediatric skeletal muscle: a promising model for juvenile dermatomyositis

Journal

RHEUMATOLOGY
Volume -, Issue -, Pages -

Publisher

OXFORD UNIV PRESS
DOI: 10.1093/rheumatology/kead186

Keywords

JDM; IFN; DM; muscle biology; tissue engineering

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This study investigated the effects of type I interferon (IFN-alpha and IFN-beta) on pediatric skeletal muscle using an in vitro human skeletal muscle model. The results showed that IFN-beta decreased muscle contractile force and slowed twitch kinetics, which could be partially reversed by JAKi treatment. Additionally, IFN-alpha and IFN-beta had different effects on muscle fatigue and these effects could not be reversed by JAKi treatment. This study highlights the importance of using a three-dimensional human skeletal muscle model to study JDM pathogenesis and test novel therapeutics.
Objective To investigate pathogenic mechanisms underlying JDM, we defined the effect of type I IFN, IFN-alpha and IFN-beta, on pediatric skeletal muscle function and expression of myositis-related proteins using an in vitro engineered human skeletal muscle model (myobundle). Methods Primary myoblasts were isolated from three healthy pediatric donors and used to create myobundles that mimic functioning skeletal muscle in structural architecture and physiologic function. Myobundles were exposed to 0, 5, 10 or 20 ng/ml IFN-alpha or IFN-beta for 7 days and then functionally tested under electrical stimulation and analyzed immunohistochemically for structural and myositis-related proteins. Additionally, IFN-beta-exposed myobundles were treated with Janus kinase inhibitors (JAKis) tofacitinib and baricitinib. These myobundles were also analyzed for contractile force and immunohistochemistry. Results IFN-beta, but not IFN-alpha, was associated with decreased contractile tetanus force and slowed twitch kinetics. These effects were reversed by tofacitinib and baricitinib. Type I IFN paradoxically reduced myobundle fatigue, which did not reverse after JAKi. Additionally, type I IFN correlated with MHC I upregulation, which normalized after JAKi treatment, but expression of myositis-specific autoantigens Mi-2, melanocyte differentiation-associated protein 5 and the endoplasmic reticulum stress marker GRP78 were variable and donor specific after type I IFN exposure. Conclusion IFN-alpha and IFN-beta have distinct effects on pediatric skeletal muscle and these effects can partially be reversed by JAKi treatment. This is the first study illustrating effective use of a three-dimensional human skeletal muscle model to investigate JDM pathogenesis and test novel therapeutics.

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