Development of air-assisted atomization device for the delivery of cells in viscous biological ink prepared with sodium alginate

Skin wounds, especially large-area skin trauma, would bring great pain and even fatal risk to patients. In recent years, local autologous cell transplantation has shown great potential for wound healing and re-epithelialization. However, when the cell suspension prepared with normal saline is delivered to the wound, due to its low viscosity, it is easy to form big drops in the deposition and lose them from the wound bed, resulting in cell loss and uneven coverage. Here, we developed a novel air-assisted atomization device (AAAD). Under proper atomization parameters, 1% (w/v) sodium alginate (SA) solution carrier could be sprayed uniformly. Compared with normal saline, the run-off of the SA on the surface of porcine skin was greatly reduced. In theory, the spray height of AAAD could be set to achieve the adjustment of a large spray area of 1-12 cm(2). In the measurement of droplet velocity and HaCaT cell viability, the spray height of AAAD would affect the droplet settling velocity and then the cell delivery survival rate (CSR). Compared with the spray height of 50 mm, the CSR of 100 mm was significantly higher and could reach 91.09% +/- 1.82% (92.82% +/- 2.15% in control). For bio-ink prepared with 1% (w/v) SA, the viability remained the same during the 72-h incubation. Overall, the novel AAAD uniformly atomized bio-ink with high viscosity and maintained the viability and proliferation rate during the delivery of living cells. Therefore, AAAD has great potential in cell transplantation therapy, especially for large-area or irregular skin wounds.

Automated summary

Skin wounds, especially large-area trauma, can cause severe pain and even be life-threatening. Local autologous cell transplantation has become a promising method for wound healing. However, the low viscosity of the cell suspension prepared with normal saline leads to uneven coverage and cell loss. A novel air-assisted atomization device (AAAD) was developed to overcome this issue. The AAAD can uniformly spray a high-viscosity bio-ink, maintaining cell viability and proliferation rate. This technology has great potential for cell transplantation therapy, especially in treating large-area or irregular skin wounds.