Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 120, Issue 23, Pages -Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2214652120
Keywords
Parkinson's disease; & alpha;-synuclein; zebrafish
Categories
Ask authors/readers for more resources
This study found that phosphorylation of a-Synuclein at T64 was increased in both Parkinson's disease models and human PD brains. The T64D phosphomimetic mutation led to the formation of a-Synuclein oligomers with a similar structure to those with the A53T mutation, and it induced mitochondrial dysfunction, lysosomal disorder, cell death, and neurodegeneration. These findings suggest a pathogenic role of a-Synuclein phosphorylation at T64 in Parkinson's disease.
a-Synuclein accumulates in Lewy bodies, and this accumulation is a pathological hallmark of Parkinson's disease (PD). Previous studies have indicated a causal role of a-synuclein in the pathogenesis of PD. However, the molecular and cellular mechanisms of a-synuclein toxicity remain elusive. Here, we describe a novel phosphorylation site of a-synuclein at T64 and the detailed characteristics of this post-translational modification. T64 phosphorylation was enhanced in both PD models and human PD brains. T64D phosphomimetic mutation led to distinct oligomer formation, and the structure of the oligomer was similar to that of a-synuclein oligomer with A53T mutation. Such phosphomimetic mutation induced mitochondrial dysfunction, lysosomal disorder, and cell death in cells and neurodegeneration in vivo, indicating a pathogenic role of a-synuclein phosphorylation at T64 in PD.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available