Journal
JOURNAL OF VIROLOGICAL METHODS
Volume 236, Issue -, Pages 215-220Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2016.07.024
Keywords
Variola virus; Monkeypox virus; Varicella-zoster virus; Real-time PCR
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A method of one-stage rapid detection and differentiation of epidemiologically important variola virus (VARV), monkeypox virus (MPXV), and varicella-zoster virus (VZV) utilizing multiplex real-time TaqMan PCR assay was developed. Four hybridization probes with various fluorescent dyes and the corresponding fluorescence quenchers were simultaneously used for the assay. The hybridization probes specific for the VARV sequence contained FAM/BHQ1 as a dye/quencher pair; MPXV-specific, JOE/BHQ1; VZV-specific, TAMRA/BHQ2; and internal control-specific, Cy5/BHQ3. The specificity and sensitivity of the developed method were assessed by analyzing DNA of 32 strains belonging to orthopoxvirus and herpesvirus species. (C) 2016 Elsevier B.V. All rights reserved.
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