Dynamic GOLVEN-ROOT GROWTH FACTOR 1 INSENSITIVE signaling in the root cap mediates root gravitropism

Throughout the exploration of the soil, roots interact with their environment and adapt to different conditions. Directional root growth is guided by asymmetric molecular patterns but how these become established or are dynamically regulated is poorly understood. Asymmetric gradients of the phytohormone auxin are established during root gravitropism, mainly through directional transport mediated by polarized auxin transporters. Upon gravistimulation, PIN-FORMED2 (PIN2) is differentially distributed and accumulates at the lower root side to facilitate asymmetric auxin transport up to the elongation zone where it inhibits cell elongation. GOLVEN (GLV) peptides function in gravitropism by affecting PIN2 abundance in epidermal cells. In addition, GLV signaling through ROOT GROWTH FACTOR 1 INSENSITIVE (RGI) receptors regulates root apical meristem maintenance. Here, we show that GLV-RGI signaling in these 2 processes in Arabidopsis (Arabidopsis thaliana) can be mapped to different cells in the root tip and that, in the case of gravitropism, it operates mainly in the lateral root cap (LRC) to maintain PIN2 levels at the plasma membrane (PM). Furthermore, we found that GLV signaling upregulates the phosphorylation level of PIN2 in an RGI-dependent manner. In addition, we demonstrated that the RGI5 receptor is asymmetrically distributed in the LRC and accumulates in the lower side of the LRC after gravistimulation. Asymmetric GLV-RGI signaling in the root cap likely accounts for differential PIN2 abundance at the PM to temporarily support auxin transport up to the elongation zone, thereby representing an additional level of control on the asymmetrical auxin flux to mediate differential growth of the root. GOLVEN-ROOT GROWTH FACTOR 1 INSENSITIVE signaling in lateral root cap cells plays an important role in root gravitropism by regulating PIN-FORMED2 plasma membrane abundance in Arabidopsis.

Automated summary

During root exploration, roots adapt to different conditions and interact with their environment. Asymmetric molecular patterns guide directional root growth, but the mechanisms of their establishment and dynamic regulation are not well understood. Asymmetric gradients of the phytohormone auxin are established through polarized auxin transporters, mainly during root gravitropism. GOLVEN-ROOT GROWTH FACTOR 1 INSENSITIVE signaling in lateral root cap cells plays a crucial role in root gravitropism by regulating the plasma membrane abundance of PIN-FORMED2 in Arabidopsis.