HOS1 promotes plant tolerance to low-energy stress via the SnRK1 protein kinase

Plants need to integrate internal and environmental signals to mount adequate stress responses. The NUCLEAR PORE COMPLEX (NPC) component HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENES 1 (HOS1) is emerging as such an integrator, affecting responses to cold, heat, light, and salinity. Stress conditions often converge in a low-energy signal that activates SUCROSE NON-FERMENTING 1-RELATED KINASE 1 (SnRK1) to promote stress tolerance and survival. Here, we explored the role of HOS1 in the SnRK1-dependent response to low-energy stress in Arabidopsis thaliana, using darkness as a treatment and a combination of genetic, biochemical, and phenotypic assays. We show that the induction of starvation genes and plant tolerance to prolonged darkness are defective in the hos1 mutant. HOS1 interacts physically with the SnRK1a1 catalytic subunit in yeast two-hybrid assays and in planta, and the nuclear accumulation of SnRK1a1 is reduced in the hos1 mutant. Likewise, another NPC mutant, nup160, exhibits lower activation of starvation genes and decreased tolerance to prolonged darkness. Importantly, defects in low-energy responses in the hos1 background are rescued by fusing SnRK1a1 to a potent nuclear localization signal or by sugar supplementation during the dark treatment. Altogether, this work demonstrates the importance of HOS1 for the nuclear accumulation of SnRK1a1, which is key for plant tolerance to low-energy conditions.

Automated summary

Plants need HOS1 as a signal integrator to respond to various stresses, including cold, heat, light, and salinity. Defects in HOS1 result in reduced induction of starvation genes and decreased tolerance to prolonged darkness, highlighting the importance of HOS1 for plant survival under low-energy conditions. HOS1 promotes the nuclear accumulation of SnRK1a1, which is crucial for plant tolerance to low-energy stress.