A Phytophthora infestans RXLR effector AVR8 suppresses plant immunity by targeting a desumoylating isopeptidase DeSI2

The potato's most devastating disease is late blight, which is caused by Phytophthora infestans. Whereas various resistance (R) genes are known, most are typically defeated by this fast-evolving oomycete pathogen. However, the broad-spectrum and durable R8 is a vital gene resource for potato resistance breeding. To support an educated deployment of R8, we embarked on a study on the corresponding avirulence gene Avr8. We overexpressed Avr8 by transient and stable transformation, and found that Avr8 promotes colonization of P. infestans in Nicotiana benthamiana and potato, respectively. A yeast-two-hybrid (Y2H) screen showed that AVR8 interacts with a desumoylating isopeptidase (StDeSI2) of potato. We overexpressed DeSI2 and found that DeSI2 positively regulates resistance to P. infestans, while silencing StDeSI2 downregulated the expression of a set of defense-related genes. By using a specific proteasome inhibitor, we found that AVR8 destabilized StDeSI2 through the 26S proteasome and attenuated early PTI responses. Altogether, these results indicate that AVR8 manipulates desumoylation, which is a new strategy that adds to the plethora of mechanisms that Phytophthora exploits to modulate host immunity, and StDeSI2 provides a new target for durable resistance breeding against P. infestans in potato.

Automated summary

Late blight caused by Phytophthora infestans is the most destructive disease in potatoes. The oomycete pathogen quickly evolves to defeat most known resistance genes, but the broad-spectrum and durable R8 gene provides valuable resources for potato resistance breeding. The study on the avirulence gene Avr8 showed that it promotes colonization of P. infestans in Nicotiana benthamiana and potato. The interaction between AVR8 and the desumoylating isopeptidase StDeSI2 in potato indicates a new strategy for modulating host immunity against P. infestans, and StDeSI2 becomes a potential target for breeding durable resistance in potato.