Analysis of aquaporins in northern grasses reveal functional importance of Puccinellia nuttalliana PIP2;2 in salt tolerance

To better understand the roles of aquaporins in salt tolerance, we cloned PIP2;1, PIP2;2, PIP2;3, PIP1;1, PIP1;3, and TIP1;1 aquaporins from three northern grasses varying is salt tolerance including the halophytic grass Puccinellia nuttalliana, moderately salt tolerant Poa juncifolia, and relatively salt sensitive Poa pratensis. We analysed aquaporin expression in roots by exposing the plants to 0 and 150 mM for 6 days in hydroponic culture. NaCl treatment upregulated several PIP transcripts in P. nuttalliana while decreasing PnuTIP1;1. The PnuPIP2;2 transcripts increased by about six-fold in P. nuttalliana, two-fold in Poa juncifolia, and did not change in Poa pratensis. The NaCl treatment enhanced the rate of water transport in yeast expressing PnuPIP2;2 by 56% compared with control. PnuPIP2,2 expression also resulted in a higher Na+ uptake in yeast cells compared with an empty vector suggesting that PnuPIP2;2 may have both water and ion transporting functions. Structural analysis revealed that the transport properties of PnuPIP2;2 could be affected by its unique pore characteristics, which include a combination of hourglass, cylindrical, and increasing diameter conical entrance shape with pore hydropathy of -0.22.

Automated summary

In this study, the expression of aquaporins in several northern grasses with different salt tolerance was analyzed. It was found that the aquaporin PnuPIP2;2 was significantly upregulated in the halophytic grass Puccinellia nuttalliana after NaCl treatment. Functional analysis showed that PnuPIP2;2 not only facilitated water transport, but also increased Na+ uptake in yeast cells. Structural analysis revealed the unique pore characteristics of PnuPIP2;2, which may contribute to its transport properties.