SHORT ROOT and INDETERMINATE DOMAIN family members govern PIN-FORMED expression to regulate minor vein differentiation in rice

C-3 and C-4 grasses directly and indirectly provide the vast majority of calories to the human diet, yet our understanding of the molecular mechanisms driving photosynthetic productivity in grasses is largely unexplored. Ground meristem cells divide to form mesophyll or vascular initial cells early in leaf development in C-3 and C-4 grasses. Here we define a genetic circuit composed of SHORT ROOT (SHR), INDETERMINATE DOMAIN (IDD), and PIN-FORMED (PIN) family members that specifies vascular identify and ground cell proliferation in leaves of both C-3 and C-4 grasses. Ectopic expression and loss-of-function mutant studies of SHR paralogs in the C-3 plant Oryza sativa (rice) and the C-4 plant Setaria viridis (green millet) revealed the roles of these genes in both minor vein formation and ground cell differentiation. Genetic and in vitro studies further suggested that SHR regulates this process through its interactions with IDD12 and 13. We also revealed direct interactions of these IDD proteins with a putative regulatory element within the auxin transporter gene PIN5c. Collectively, these findings indicate that a SHR-IDD regulatory circuit mediates auxin transport by negatively regulating PIN expression to modulate minor vein patterning in the grasses. A SHORT ROOT-INTERMEDIATE DOMAIN regulatory circuit modulates auxin transport by negatively regulating PIN-FORMED expression to regulate minor vein patterning in grasses.

Automated summary

C-3 and C-4 grasses are crucial for human diet, but our understanding of the molecular mechanisms driving photosynthesis in these grasses is limited. A genetic circuit involving SHR, IDD, and PIN family members controls vein formation and ground cell proliferation in leaves of both C-3 and C-4 grasses. This circuit regulates auxin transport through negative regulation of PIN expression and plays a role in minor vein patterning in grasses.