Journal
PHYTOCHEMISTRY
Volume 210, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.phytochem.2023.113643
Keywords
Euphorbia wallichii Hook; f; Euphorbiaceae; Ent-kauranes; Ent-atisanes; Anti-inflammatory activity
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Phytochemical investigation on Euphorbia wallichii led to the discovery of twelve diterpenoids, including nine previously undescribed compounds. These compounds showed potent inhibitory effects on NO production in LPS-induced RAW264.7 cells, with wallkaurane A being the most active (IC50 = 4.21 mu M). Mechanistic studies revealed that wallkaurane A could suppress the generation of pro-inflammatory cytokines, decrease the expression of iNOS and COX-2, and regulate the NF-kappa B and JAK2/STAT3 signaling pathways to suppress inflammation and apoptosis.
Phytochemical investigation on the whole plant of Euphorbia wallichii led to the identification of twelve diter-penoids, including nine undescribed ones, in which wallkauranes A-E (1-5) were classified as ent-kaurane diterpenoids and wallatisanes A-D (6-9) were assigned as ent-atisane diterpenoids. The biological evaluation of these isolates against NO production was conducted in the LPS-induced RAW264.7 macrophage cells model, resulting in the identification of a series of potent NO inhibitors, with the most active wallkaurane A showing an IC50 value of 4.21 mu M. The mechanistic study disclosed that wallkaurane A could inhibit pro-inflammatory cy-tokines generation such as TNF-alpha, IL-1 beta, and IL-6, and decrease the expression of iNOS and COX-2. Wallkaurane A could regulate the NF-kappa B signaling pathways and the JAK2/STAT3 signaling pathway to suppress the in-flammatory reaction in LPS-induced RAW264.7 cells. Meanwhile, wallkaurane A could also inhibit the JAK2/ STAT3 signaling pathway, thereby suppressing apoptosis in LPS-induced RAW264.7 cells.
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