Resistant risk and resistance-related point mutation in SdhC1 of pydiflumetofen in Fusarium pseudograminearum

BACKGROUNDFusarium pseudograminearum is one of the dominant pathogens of Fusarium crown rot (FCR) worldwide. Unfortunately, no fungicides have yet been registered for the control of FCR in wheat in China. Pydiflumetofen, a new-generation succinate dehydrogenase inhibitor, exhibits excellent inhibitory activity to Fusarium spp. A resistance risk assessment of F. pseudograminearum to pydiflumetofen and the resistance mechanism involved have not yet been investigated. RESULTSThe median effective concentration (EC50) value of 103 F. pseudograminearum isolates to pydiflumetofen was 0.0162 & mu;g mL(-1), and the sensitivity exhibited a unimodal distribution. Four resistant mutants were generated by fungicide adaption, which possessed similar or impaired fitness compared to corresponding parental isolates based on the results of mycelial growth, conidiation, conidium germination rate, and virulence determination. Pydiflumetofen showed strong positive cross-resistance with cyclobutrifluram and fluopyram but no cross-resistance with carbendazim, phenamacril, tebuconazole, fludioxonil, or pyraclostrobin. Sequence alignment revealed that pydiflumetofen-resistant F. pseudograminearum mutants had two single-point mutations of A83V or R86K in FpSdhC(1). Molecular docking further confirmed that point mutation of A83V or R86K in FpSdhC(1) could confer resistance of F. pseudograminearum to pydiflumetofen. CONCLUSIONFusarium pseudograminearum shows an overall moderate risk of developing resistance to pydiflumetofen, and point mutation FpSdhC(1)(A83V) or FpSdhC(1)(R86K) could confer pydiflumetofen resistance in F. pseudograminearum. This study provided vital data for monitoring the emergence of resistance and developing resistance management strategies for pydiflumetofen. & COPY; 2023 Society of Chemical Industry.

Automated summary

This study investigated the resistance risk of Fusarium pseudograminearum to the succinate dehydrogenase inhibitor pydiflumetofen and the resistance mechanism involved. It was found that F. pseudograminearum showed a moderate risk of developing resistance to pydiflumetofen, and point mutations in FpSdhC(1) could confer pydiflumetofen resistance.