High-throughput Sequencing to Identify Bacteremia in Children with Cancer

Current methods to diagnose bacteremia are limited. In this pilot study of children with cancer presenting with fever, we determined the concordance between a novel high-throughput sequencing platform called BacCapSeq and blood culture. High-throughput sequencing had modest concordance with blood culture. Discordant organisms included those with both unlikely or potential clinical relevance.

Automated summary

In this pilot study, we found that a novel high-throughput sequencing platform, called BacCapSeq, had moderate agreement with blood culture in diagnosing bacteremia among children with cancer presenting with fever. There were discordant organisms, some of which were clinically irrelevant.