Journal
NEUROINFORMATICS
Volume 21, Issue 2, Pages 269-286Publisher
HUMANA PRESS INC
DOI: 10.1007/s12021-023-09623-9
Keywords
Light sheet fluorescence microscopy; Magnetic resonance imaging; Brain atlas; Whole brain imaging; Multimodal imaging; Crossmodal registration
Ask authors/readers for more resources
Magnetic resonance imaging (MRI) and light-sheet fluorescence microscopy (LSFM) are technologies that allow non-invasive imaging of whole mouse brains in three dimensions. This study developed a bidirectional multimodal atlas framework to facilitate the translation of LSFM recorded brains to non-distorted templates, improving research on neuroscience, disease progression, and drug efficacy.
Magnetic resonance imaging (MRI) and light-sheet fluorescence microscopy (LSFM) are technologies that enable non-disruptive 3-dimensional imaging of whole mouse brains. A combination of complementary information from both modalities is desirable for studying neuroscience in general, disease progression and drug efficacy. Although both technologies rely on atlas mapping for quantitative analyses, the translation of LSFM recorded data to MRI templates has been complicated by the morphological changes inflicted by tissue clearing and the enormous size of the raw data sets. Consequently, there is an unmet need for tools that will facilitate fast and accurate translation of LSFM recorded brains to in vivo, non-distorted templates. In this study, we have developed a bidirectional multimodal atlas framework that includes brain templates based on both imaging modalities, region delineations from the Allen's Common Coordinate Framework, and a skull-derived stereotaxic coordinate system. The framework also provides algorithms for bidirectional transformation of results obtained using either MR or LSFM (iDISCO cleared) mouse brain imaging while the coordinate system enables users to easily assign in vivo coordinates across the different brain templates.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available